β-Mercaptoethanol-modified ELISA for diagnosis of visceral leishmaniasis

A Capture ELISA for the Diagnosis of Visceral Leishmaniasis Using a Monoclonal Antibody against a Leishmanial

capture ELISA system was developed for diagnosis of visceral leishmaniasis (VL) using a monoclonal antibody raised against an antigen previously detected in the urine of VL patients. Urine samples from confirmed VL cases from Yemen, Nepal, Spain, Sudan and Brazil were tested in the capture ELISA in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...

Comparison of Serological Methods (ELISA, DAT and IFA) for Diagnosis of Visceral Leishmaniasis Utilizing an Endemic Strain

Background: The causative agent of visceral leishmaniasis (VL) in Iran is Leishmania infantum (L. infantum) (Mediterranean type) and its major reservoir host is the dog. Ob-jective: To compare the serological methods including direct agglutination test (DAT), indirect immunofluorescent-antibody test (IFA) and enzyme-linked immunosorbent as-say (ELISA) for serodiagnosis of endemic strain of L. i...

[Diagnosis of visceral leishmaniasis].

The differential diagnosis between visceral leishmaniasis and lymphoplasmacell polimorphocell lymphosarcoma may be difficult. One case is described in which electron microscopy allowed detection of Leishmania in the histiocytes of the spleen.

Diagnosis of visceral leishmaniasis.

Leishmaniasis is a vector-borne disease with up to 350 million people at risk of infection worldwide. Among its different clinical manifestations, visceral is the most severe form. Since clinical features of visceral leishmaniasis (VL) mimic several other common diseases, accurate diagnosis is crucial as the treatment is associated with significant toxicity. Invasive and risky techniques involv...

Laboratory diagnosis of visceral leishmaniasis.